The Letran Knights are the collegiate varsity teams representing Colegio de San Juan de Letran in the National Collegiate Athletic Association. The collegiate women's varsity teams are called the Lady Knights, while the high school varsity teams are called the Squires.
The college's varsity teams also participate in other sports leagues such as the Filoil Flying V Preseason Cup, Fr. Martin's Cup, Premier Volleyball League, Milcu Got Skills Inter School, among others.
Letran College had organized its own football team in 1910, known as the Letran Athletic Association. The team, nicknamed "The Bohemians", won the national championship in the years up to 1917. Team was led by Joaquín Loyzaga Sr. and Letran Football Hall of Famer, Joaquin Lopez. Then, it joined the La Liga Catolica where it competed against other Catholic schools. The Letran Booters joined the NCAA in 1932.
The Letran Booters won several titles in the NCAA, most notably during the 1980s, when they beat a favored Mapua team with Taiwanese and foreign-bred players.
In season 2002, its juniors team, the Letran Squires, won the NCAA title in a clean sweep manner, beating everyone and winning all of their matches. The title was won in dramatic fashion, as the final match against PCU went into extra time, and Letran scoring the golden goal to clinch the title. During this season, the Letran Squires only managed to concede one goal in the whole competition. This team was coached by Bernie Cordero and Perival Acabado and a double MVP award were given to Bobby Durano (Forward) and Bryan Alvarez (Attacking Midfield).
Due to financial restraints, Letran College discontinued the seniors football program in 2003. In 2018, the seniors football team was re-established.